mouse anti human CD326:Low Endotoxin
货号:MCA1870EL
规格:0.5 mg
价格:5100
产品类型:流式抗体
品牌:Bio-Rad
抗原:CD326
物种:人
宿主:小鼠
抗体亚型:IgG1, kappa
克隆号: VU-1D9
荧光染料:其它
产品介绍 | | |
Mouse anti Human CD326 antibody, clone VU-1D9recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, andde novoexpression being seen in dysplastic squamous epithelium. |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Carrier Free | Yes |
Immunogen | HG9 cell line (small cell lung carcinoma) |
Approx. Protein Concentrations | 1 mg/ml |
Fusion Partners | Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
存储条件 | | |
Store at -20oC only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/10 | 1/200 |
Immunohistology - Frozen | √ | | |
Immunohistology - Paraffin1 | √ | | |
Immunoprecipitation | √ | | |
Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
- Histology Positive Control TissueLarge Bowel
|
同型 |
Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | Flow | 0.5 mg |
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Mouse Seroblock FcR | BUF041B | Flow | 0.5 mg |
文献 |
References for CD326 antibody1. Koga, Y.et al.(2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.Gastroenterol Res Pract. 2008: 605273.2. Fillmore, C.M.et al.(2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.Proc Natl Acad Sci U S A. 107 (50): 21737-42.3. Murakata, A.et al.(2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.Ann Surg. 253: 94-100.4. Keller, P.J.et al.(2012) Defining the cellular precursors to human breast cancer.Proc Natl Acad Sci U S A. 109 (8): 2772-7.5. Jeong, H.T.et al.(2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.Liver Int. 32: 430-40.6. Armstrong, A.J.et al.(2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.PLoS One. 14 (5): e0216934.Further Reading7. Winter, M.J.et al.(2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.Am J Pathol. 163 (6): 2139-48. |
技术参数 产品应用 IHC(F); Flow; IP ;IHC(P) * ;WB
产品类型 Monoclonal Antibody
推荐用于 IHC(F); Flow; IP ;IHC(P) * ;WB
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