| 产品介绍 | | |
| Mouse anti Horse IgGb antibody, clone CVS39recognizes equine IgGb, also known as equine IgG4/7 (Kegganet al.2013), and shows no significant binding to other IgG subclasses. Equine IgGb is an immunologlobulin with a molecular weight (MW) of approximately 160kDa, the MW of the light chain being 27kDa, common to all equine IgG subclasses, while the heavy chain has a MW of 53kDa, specific to IgGb (Sheoranet al.1998). Equine IgGb has been demonstrated to be involved in long lasting immunity following respiratory-related viral infections such as Equine Herpes virus Type 4 (EHV-4) (Mizukoshiet al.2002) and Equine Influenza (Sobollet al.2003).In addition to Mouse anti Horse IgGb, clone CVS39, Bio-Rad also offer a range of other monoclonal and polyclonal antibodies that are specific to equine immunoglobulin classes and subclasses. |
| 产品详情 | | |
| Target Species | Horse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Purified equine IgGb. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/5000 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Sheoran, A.S.et al.(1998) Monoclonal antibodies to subclass-specific antigenic determinants on equine immunoglobulin gamma chains and their characterization.Vet Immunol Immunopathol. 62 (2): 153-65.2.Lewis, M.J.et al.(2008) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.Mol Immunol. 45 (3): 818-27.3.Keggan, A.et al(2013) Production of seven monoclonal equine immunoglobulins isotyped by multiplex analysisVet Immunol Immunopathol.153(3-4):187-934.Palm, A.E.et al.(2016) Secretory immunoglobulin A and immunoglobulin G in horse saliva.Vet Immunol Immunopathol. 180: 59-65. |
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