Mouse anti Horse Pan B-Cells:RPE
货号：MCA1899PE
规格：100 Tests
价格：4352
产品类型：流式抗体
品牌：Bio-Rad
抗原：Pan B-Cells
物种：Horse
宿主：小鼠
抗体亚型：IgG1
克隆号：CVS36
荧光染料：PE
| 产品介绍 | | |
| Rat anti Mouse CD115 antibody, clone 604B5 2E11recognizes the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. Murine CD115 is a 958 amino acid 110kDa single pass type I transmembrane glycoprotein with tyrosine kinase activity and acts as a membrane receptor for M-CSF.CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Rat anti Mouse CD115 antibody, clone 604B5 2E11 has been shown to inhibitin vitrocolony formation in response to M-CSF in both rats and mice. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line. |
| Immunogen | Purified Equine Ig. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Schneider, R. (2010) Analysis of antibody subtypes and T lymphocyte activation following vaccination of healthy foals against West Nile VirusIn M.Sc thesis University of Pennsylvania, Chapter 3, p.34-452.Lunn, D.P.et al.(1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.Vet Immunol Immunopathol. 62 (2): 101-43.3.Umlauf, C. (2004) Herstellung und Charakterisierung monoklonaler Antikörper gegen equine LeukozytenIn Phd thesis Ludwig-Maximillians-Universität München4.Tomlinson, J.E.et al.(2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.Vet Immunol Immunopathol. 199: 22-31.5.Cequier, A.et al.(2022) Equine Mesenchymal Stem Cells Influence the Proliferative Response of Lymphocytes: Effect of Inflammation, Differentiation and MHC-Compatibility.Animals (Basel). 12 (8): 984. |
技术参数 产品应用 Elisa；Flow
产品类型 Monoclonal Antibody
推荐用于 Elisa；Flow
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