| 产品介绍 | | |
| Mouse anti Dog CD11d antibody, clone CA11.8H2recognizes the canine CD11d cell surface antigen, also known as integrin alpha d subunit. The alpha d integrin (CD11d) subunit is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp.Over expression of CD11d is noted in histiocytic disorders including hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages (Moore.et al.2006) with macrophages demonstrating markederythrocytic phagocytosis. Previously, canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with canine hepatosplenic T cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp. CD11+ γ/δ T cells constitute ~30% red pulp T cells despite their rarity in peripheral blood, suggesting a splenic red pulp origin of HTSL (Fryet al.2003). |
| 产品详情 | | |
| Target Species | Dog |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Affinity purified CD11 antigen from canine spleen. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/50 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Danilenko, D.M.et al. (1995) A novel canine leukointegrin, alpha d beta 2, is expressed by specific macrophage subpopulations in tissue and a minor CD8+ lymphocyte subpopulation in peripheral blood.J Immunol. 155:35-44.2.Moore, P.F.et al.(2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.Vet Pathol. 43:632-45.3.Vernau, W. and Moore, P.F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.Vet Immunol Immunopathol. 69:145-64. |
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