| 产品介绍 | | |
| Rabbit anti Human ORAI1 antibodyrecognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family.Antigen stimulation of immune cells triggers Ca2+entry through Ca2+release-activated Ca2+(CRAC) channels. CRAC channels are the main pathway for Ca2+influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+sensor.Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+entry.Rabbit anti Human ORAI1 antibody ( AHP1494) is not expected to cross react with either ORAI2 or ORAI3. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human ORAI1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | A 16 amino acid peptide from near the carboxy terminus of human ORAI1. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | |
Western Blotting | √ | 0.5 | 1ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1494 detects a band of approximately 49kDa in 49 cell lysates.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Western blot analysis of human ovary probed with Rabbit anti Human ORAI1 (AHP1494) at 0.5(A), 1(B) and 2(C) μg/ml |
| 文献 |
| 1. Feske, S.,et al. (2006) A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function.Nature; 441:179-852. Soboloff, J.et al.(2006) Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication.Biochim Biophys Acta. 1763 (11): 1161-8.3. Lewis, R.S. (2001) Calcium signaling mechanisms in T lymphocytes.Annu Rev Immunol. 19: 497-521. |
QQ:1749072012