| 产品介绍 | | |
| Rabbit anti Human TACR3 antibodyrecognizes human TACR3 otherwise known as NK3 Receptor (NK3R), a 52.2 kDa multi-pass membrane protein belonging to the G-protein coupled receptor 1 family.TACR3 is a receptor for the tachykinin neuropeptide, neuromedin-k otherwise known as neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system, and it is likely that these signals lead to the release of vasopressin and oxytocin into the circulation. TACR3 is involved in learning and memory (Zhanget al. 2020).Rabbit anti Human TACR3 antibody (AHP1492) does not recognize NK1R or NK2R. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human TACR3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | An 18 amino acid peptide from near the centre of human TACR3. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | 5ug/ml |
Western Blotting | √ | 0.5 | 1.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human brain
- Western Blotting
- AHP1492 detects a band of approximately 46kDa in RAW264.7 cell lysates.
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| 数据 |
Formalin fixed, paraffin embedded human brain with Rabbit anti Human TACR3 (AHP1492) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0). |
| 文献 |
| 1. Haley, G.E. & Flynn, F.W. (2007) Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge.Am J Physiol Regul Integr Comp Physiol. 293 (2): R931-7.2. Ding, Y.Q.et al.(1999) Neurokinin B receptor (NK3)-containing neurons in the paraventricular and supraoptic nuclei of the rat hypothalamus synthesize vasopressin and express Fos following intravenous injection of hypertonic saline.Neuroscience. 91 (3): 1077-85.3. Maggi, C.A. (1995) The mammalian tachykinin receptors.Gen Pharmacol. 26 (5): 911-44.4. Nakajima, Y.et al.(1992) Direct linkage of three tachykinin receptors to stimulation of both phosphatidylinositol hydrolysis and cyclic AMP cascades in transfected Chinese hamster ovary cells.J Biol Chem. 267 (4): 2437-42. |
QQ:1749072012