Rabbit anti Human Delta-like Protein 4 antibody
货号：AHP1274
规格：0.1 mg
价格：4888
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Delta-like Protein 4
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human Delta-like Protein 4 antibodydetects human Delta-like protein 4 (DLL4), also known as Delta-4. DLL4 is a 685 amino acid single pass type 1 transmembrane glycoprotein of ~72 kDa containing a singleDSLdomain and eightEGF-likedomains. Human DLL4 is the homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor, Notch1.DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network (Mailhoset al.2001). DLL4 has been found to be strongly expressed in tumour vessels of primary renal tumours (Patelet al.2005) and bladder cancer (Patelet al.2006), and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumour growth, with no apparent toxicity (Ridgwayet al.2006).Expression of DLL4 in normal human dermis is low in foetal tissues, becomes more intense during early life (0-20 years) and gradually declines thereafter as shown by immunohistochemical studies on FFPE tissues using rabbit anti human DLL4 antibody (Guninet al.2014). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antisera to DLL4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Synthetic peptide corresponding to the internal region of human DLL4. |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/300 | 1/1500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.You, C.et al.(2013) Loss of CCM3 impairs DLL4-Notch signalling: implication in endothelial angiogenesis and in inherited cerebral cavernous malformations.J Cell Mol Med. 17 (3): 407-18.2.Villaamil, V.M.et al.(2012) Multiple biomarker tissue arrays: A computational approach to identifying protein-protein interactions in the EGFR/ERK signalling pathway.J Mol Signal. 7: 14.3.Gunin, A.G.et al.(2014) Age-related changes in angiogenesis in human dermis.Exp Gerontol. 55C: 143-51. |
技术参数 产品应用 elisa；wb
产品类型 Polyclonal Antibody
推荐用于 elisa；wb
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