| 产品介绍 | | |
| Rabbit anti Human muscarinic receptor M3 antibodydetects the muscarinic receptor M3, a G protein-coupled receptor for acetylcholine which is expressed in smooth muscle and glandular tissues. The M3 subtype is coupled to other G proteins known as Goor Gqwhich activate phospholipase C leading to an increase in intracellular Ca2+. The M3 muscarinic receptor controls smooth muscle contraction in blood vessels and lungs as well as in the gastrointestinal tract. It is also located in many glands, and its activation helps to stimulate secretion.Mutations in the CHRM gene can lead to the development of Prune Belly Syndrome (PBS), a condition characterized by weak abdominal musculature, lax overlying skin, cryptorchism and urinary tract abnormalities (Weberet al.2011). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human muscarinic receptor M3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Immunogen | Synthetic peptide corresponding to the 3rd cytoplasmic domain of human muscarinic receptor M3, conjugated to Keyhole Limpet Hemocyanin (KLH) |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | 10ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Brain, cortex.
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| 数据 |
Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human muscarinic receptor m3 (AHP1355) |
| 文献 |
| References for Muscarinic Receptor M3 antibody1. Billington, C.K. & Penn, R.B. (2002) m3 muscarinic acetylcholine receptor regulation in the airway.Am J Respir Cell Mol Biol. 26 (3): 269-72.Further Reading1. Felton, J.et al.(2018) Targeting M3 Muscarinic Receptors for Colon Cancer Therapy.Curr Mol Pharmacol. Jan 18 [Epub ahead of print].2. Pronin, A.N.et al.(2017) Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor.Mol Pharmacol. 92 (5): 601-612. |
QQ:1749072012