| 产品介绍 | | |
| Rabbit anti Human CD5l antibodydetects the C-terminal (CT) region of human CD5 like (CD5L), also known as Apoptosis Inhibitor expressed by Macrophages (AIM), and SP alpha. CD5L is a soluble ~38 kDa glycoprotein produced by mature macrophages, and has been shown to support their survival and enhance phagocytic function. CD5L expression is upregulated in response to LXR/RXR heterodimer activation.CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human CD5L were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | 13 amino acid peptide sequence near the carboxy terminus of human CD5L. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | |
Western Blotting | √ | 0.5 | 1.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1168 detects a band of approximately 38kDa in Raji cell lysates.
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| 二抗相关产品 |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Formalin fixed, paraffin embedded human lymph node stained with Rabbit anti Human CD5L (AHP1168) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0). |
| 文献 |
| References for CD5L antibody1. Li, Y.et al.(2008) Extracellular Nampt promotes macrophage survival via a nonenzymatic interleukin-6/STAT3 signaling mechanism.J Biol Chem. 283 (50): 34833-43.Further Reading1. Sarrias, M.R.et al.(2005) A role for human Sp alpha as a pattern recognition receptor.J Biol Chem. 280 (42): 35391-8.2. Arai, S.et al.(2005) A role for the apoptosis inhibitory factor AIM/Spalpha/Api6 in atherosclerosis development.Cell Metab. 1 (3): 201-13. |
QQ:1749072012