Goat anti Human CD14 antibody
货号：AHP1059
规格：0.1 mg
价格：6095
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：CD14
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti Human CD14 antibodyrecognizes human CD14, a 55 kDa receptor for endotoxin and other bacterial cell wall components.Goat anti Human CD14 antibody detects an epitope within the internal region of CD14. CD14 acts as a multiligand pattern recognition receptor, inducing specific coassembly of additional receptors, including MD-2 and TLR4 as part of the lipopolysaccharide (LPS) receptor.The LPS receptor is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, and is also expressed by most tissue macrophages. Activation of the LPS receptor leads to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 also up-regulates cell surface molecules, including adhesion molecules, and has been shown to bind apoptotic cells |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG- liquid |
| Antiserum Preparation | Antisera to CD14 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using the immunizing peptide, reactivity with the carrier protein was removed during purification. |
BufferSolution | TRIS buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide (NaN3) 0.5%Bovine Serum Albumin |
Immunogen | Peptide with sequence C-KRVDADADPRQYAD corresponding to amino acid residues 71-84 of the human CD14 precursor conjugated to a carrier protein. |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/128000 | |
| Immunohistology - Paraffin1 | √ | 4 | 6 ug/ml |
| Western Blotting | √ | 1 | 3 ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Citrate buffer, pH6.0, is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human Spleen
- Western Blotting
- AHP1059 detects a band of approximately 55kD in human lung, lymph node and tonsil lysates.
|
| 文献 |
| 1. Triantafilou, M. & Triantafilou, K. (2002) Lipopolysaccharide recognition: CD14, TLRs and the LPS-activation cluster.Trends Immunol. 23 (6): 301-4.2. Miyake, K. (2003) Innate recognition of lipopolysaccharide by CD14 and toll-like receptor 4-MD-2: unique roles for MD-2.Int Immunopharmacol. 3 (1): 119-28. |
技术参数 产品应用 E;P*;WB
产品类型 Polyclonal Antibody
推荐用于 E;P*;WB
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