| 产品介绍 | | |
| Sheep anti Green Fluorescent Protein antibodyrecognizes green fluorescent protein (GFP), a ~27 kDa protein derived from the jellyfishAequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression. |
| 产品详情 | | |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 5.0 mg/ml |
Immunogen | Green fluorescent protein fromAequorea victoria. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Immunofluorescence | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C;E;F;WB | 1.5 ml |
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F;IF | 0.1 mg |
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C;E;P;WB | 1 ml |
| 数据 |
Published customer image:Sheep anti green fluorescent protein antibody (4745-1051) used for the detection of GFP tagged protein by immunofluorescence.Image caption:Khc is dispensable for early eye pattern formation. (A) A null mutant, khc8, was utilized to generate mutant clones in developing third-instar larval eye discs. Crb (apical marker, red) and E-cad (AJ maker, blue) showed little defects in third-instar larval eye discs. (B) A magnified view of (A). Mutant clones were marked by the absence of GFP (green). |
| 文献 |
| 1. Collins, R.T.et al.(2010) MAZe: a tool for mosaic analysis of gene function in zebrafish.Nat Methods. 7: 219-23.2. Wu, L.et al.(2011) Properties of a distinct subpopulation of GABAergic commissural interneurons that are part of the locomotor circuitry in the neonatal spinal cord.J Neurosci. 31 (13): 4821-33.3. Knipe, L.et al.(2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.Blood. 116: 2183-91.4. Shneider, N.A.et al.(2009) Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival.Neural Dev. 4: 42.5. Soza-Ried, C.et al.(2008) Maintenance of thymic epithelial phenotype requires extrinsic signals in mouse and zebrafish.J Immunol. 181: 5272-7.6. Lopez, K.A.et al.(2011) Convection-enhanced delivery of topotecan into a PDGF-driven model of glioblastoma prolongs survival and ablates both tumor-initiating cells and recruited glial progenitors.Cancer Res. 71: 3963-71. |
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