Native Mouse Collagen I/III
货号：2150-2506
规格：10 mg
价格：2635
产品类型：蛋白
品牌：Bio-Rad
| 产品介绍 | | |
| Murine collagen type I 45%Murine collagen type III 45%Murine collagen type IV 10%Murine collagen type V <1%Non-collagenous proteins <0.5%M[a1(I)1a2(I)2]. Native triple helix. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified protein from murine tail tendons - lyophilized |
Preparation | Pepsin treatment, acetic acid extraction, serial salt precipitations |
Buffer Solution | Essentially salt free |
Preservative Stabilisers | None present |
Reconstitution | Use 0.05-0.5 M acetic acid, pH 2.5 at 4ºC. Dissolved collagen retains immunologic properties of native collagen types I+III. Thermal denaturation converts dissolved collagen to gelatin. |
Purity | Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement and ability to form microfibrils. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
Protein Molecular Weight | 300 kDa |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Rhodes, R.K. & Miller, E.J. (1978) Physicochemical characterization and molecular organization of the collagen A and B chains.Biochemistry. 17 (17): 3442-8. |
技术参数 产品应用 ELISA
产品类型 Purified Protein
推荐用于 ELISA
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