| 产品介绍 | | |
| Rabbit anti Rat Collagen I antibodyrecognizes both native and heat denatured rat collagen type I. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (~95 kDa) is found in bone, cornea, skin and tendon.Mutations in the encoding gene are associated with osteogenesis imperfecta (Sykeset al.1990), Ehlers Danlos syndrome (Burrowset al.1996), and idiopathic osteoporosis (Pernowet al.2006).Bio-Rad currently do not support the use Rabbit anti Rat Collagen I antibody in western Blotting. However, it has been reported that this antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting (Valero-Muñozet al.2014). |
| 产品详情 | | |
Target Species | Rat |
Product Form | Purified IgG - lyophilized |
Preparation | Purified IgG prepared by affinity chromatography on immobilized rat collagen I |
Buffer Solution | 0.1M Sodium Chloride 0.01M Sodium Phosphate0.01M Sodium Borate |
Preservative Stabilisers | 1% Mannitol1% Dextran |
Reconstitution | Reconstitute with 500 µl of sterile DI water. Vial contains 0.05 mg of lyophilized antibody. |
Immunogen | Type I Collagen from rat tail tendon. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/1500 | 1/6000 |
Immunofluorescence | √ | 1/10 | 1/60 |
| Immunohistology - Paraffin | √ | | |
Immunohistology - Frozen | √ | 1/10 | 1/60 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.ImmunohistologyIt has been reported that this antibody is suitable for use on paraffin embedded formalin fixed sections following treatment with 1 mg/ml pepsin in 0.5 M acetic acid for 2 hours at 37ºC and multiple buffer washes. |
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| 数据 |
Published customer image:Rabbit anti Rat collagen I antibody used for demonstration of collagen I expression in rat kidney by immunohistochemistry on paraffin embedded tissue sections.Image caption:Renin, collagen and PCNA stainings in renal tissue.Representative photomicrographs of renin, collagen I and PCNA staining in the kidneys of experimental groups. Sham=sham operated controls, NMH=non-malignant hypertension, MH=malignant hypertension, neg. control=IgG control staining for specificity of antibody. |
| 文献 |
| 1. Daniel, C.et al.(2009) Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation.Am J Physiol Renal Physiol. 297: F1299-309.2. Hartner A,et al. (2006) Angiotensin II formation in the kidney and nephrosclerosis in Ren-2 hypertensive rats.Nephrol Dial Transplant. 21:1778-85.3. Klanke B,et al. (2008) Blood pressure versus direct mineralocorticoid effects on kidney inflammation and fibrosis in DOCA-salt hypertension.Nephrol Dial Transplant. 23: 3456-63.4. Jones, H.B.et al.(2012) Quantitative Histopathological Assessment of Retardation of Islets of Langerhans Degeneration in Rosiglitazone-dosed Obese ZDF Rats Using Combined Insulin and Collagens (I and III) Immunohistochemistry with Automated Image Analysis and Statistical Modeling.Toxicol Pathol. 41: 425-44.5. Valero-Muñoz, M.et al.(2014) Carob pod insoluble fiber exerts anti-atherosclerotic effects in rabbits through sirtuin-1 and peroxisome proliferator-activated receptor-γ coactivator-1α.J Nutr. 144: 1378-84. |
QQ:1749072012