| 产品介绍 | | |
| Rabbit anti Mouse Collagen IV antibodyrecognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28 kDa arresten fragment, collagen α2(IV) yields a c-terminal canstatin fragment while Collagen α3(IV) yeilds a tumstatin fragment. Collagen IV bears a singlecollagen IV NC1(C-terminal non-collagenous) domain (UniProt: Q9QZR9).Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisieret al.2007).Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tanget al.2010), rats (Shenet al.2014) and oragutan (Bredieset al.2013).The following cross reactivities have been observed:Mouse type IV100%Mouse types I, II & III<0.1%Human types IV & V<0.1%Mouse fibronectin & laminin<0.1% |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified Ig - liquid |
Preparation | Purified IgG prepared by antigen column chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3)Antibiotic antimycotic mixture 1% |
Immunogen | Collagen IV purified from mouse EHS tumor. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/2000 | |
| Immunofluorescence | √ | 1/40 | |
Immunohistology - Paraffin | √ | 1/500 | |
Immunohistology - Frozen | √ | 1/500 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
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Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Published customer image:Rabbit anti Mouse Collagen IV antibody (2150-1470) used to label blood vessels in mouse dental tissue by immunofluorescence.Image caption:Immunohistochemistry-based validation of AIF1+, LYVE1+ macrophages inside themouse incisor. Together with the figure 5c these panels prove the different histological positioning of two different kind of macrophages (LYVE1+ and LYVE1-). AIF1+ macrophages are located in the whole incisor including apical pulp, cervical loop, odontoblast layer and distal pulp in contrast to LYVE+ macrophages which mostly resides in the middle part of the pulp, but not inside the odontoblast layer.COL4 immunohistochemical staining to visualize the blood vessels. |
| 文献 |
| 1. Armulik, A.et al.(2010) Pericytes regulate the blood-brain barrier.Nature. 468: 557-61.2. Xu Q.et al. (2004) Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair.Cell. 116: 883-95.3. Tang, Z.et al.(2010) Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.J Exp Med. 207: 867-80.4. Fantin, A.et al.(2010) Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF-mediated endothelial tip cell induction.Blood.116: 829-40.5. Shen, W.et al.(2014) Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.PLoS One. 9: e104759. |
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