Mouse anti-Rat IgG2a Secondary Antibody, PE小鼠抗大鼠IgG2a 二抗, PE
货号:12-4817-82
规格:100μg
价格:1649
产品类型:荧光二抗
品牌:Thermo Fisher
物种:大鼠
宿主:小鼠
抗体亚型:IgG
克隆号:r2a-21B2
荧光染料:PE
类型: | 单抗 | 同型对照: | IgG1, kappa |
浓度: | 0.2 mg/mL | 用法: | 0.06 µg/test(Flow) |
产品详细信息描述:单克隆抗体r2a-21B2识别大鼠IgG2a抗体,可用作流式细胞术和显微镜检查的第二步试剂。单克隆不能识别其他大鼠同种型,也不能与小鼠IgG2a或任何小鼠同型抗体发生交叉反应。报告的应用:已报道该R2a-21B2抗体可用于流式细胞仪分析。测试的应用程序:此R2a-21B2抗体已通过流式细胞仪分析了用大鼠IgG2a一抗染色的细胞。每次测试可使用小于或等于0.06 µg的水。测试的定义是将以100 µL的最终体积对细胞样品染色的抗体量(µg)。细胞数应根据经验确定,但范围为10 ^ 5至10 ^ 8个细胞/测试。建议在目标分析中仔细滴定抗体以获得最佳性能。激发:488-561 nm;发射:578nm;激光:蓝色激光,绿色激光,黄绿色激光。过滤:制造后0.2 µm过滤。靶标信息抗大鼠第二抗体是具有亲和力的抗体,对大鼠免疫球蛋白具有良好的特异性,可用于检测,分选或纯化其特定靶标。二抗提供了更多的多功能性,使用户能够使用许多检测系统(例如HRP,AP,荧光)。它们还可以通过信号放大来提供更高的灵敏度,因为多个二级抗体可以与单个一级抗体结合。最常见地,通过用来自靶物种的免疫球蛋白的汇集群体对宿主动物进行免疫来产生二抗,并且可以进一步纯化和修饰(即免疫亲和层析,抗体片段化,标记缀合等)以产生高度特异性的试剂。
数据 |
大鼠IgG2a二抗(12-4817-82)流式图用抗小鼠CD8a APC(克隆53-6-7,大鼠IgG2a同种型)(产品编号17-0081-82)对C57Bl / 6脾细胞进行染色,然后将0.03 µg抗大鼠IgG2a PE染色(右)。 将总活细胞用于分析。 |
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二抗荧光二抗免疫组化一抗标签抗体 |
参考文献: |
1.Journal of immunology (Baltimore, Md. : 1950)Histone Deacetylase 3 Is Required for T Cell Maturation."12-4817 was used in Flow cytometry/Cell sorting to examine whether HDAC3 is also required for T cell maturation."AuthorsHsu FC,Belmonte PJ,Constans MM,Chen MW,McWilliams DC,Hiebert SW,Shapiro VS2.The Journal of clinical investigationApoptotic cells trigger a membrane-initiated pathway to increase ABCA1."12-4817 was used in Flow cytometry/Cell sorting to identify a membrane-initiated pathway that is triggered by apoptotic cells to enhance ABCA1 within engulfing phagocytes."AuthorsFond AM,Lee CS,Schulman IG,Kiss RS,Ravichandran KS3.Scientific reportsThe anti-fibrotic effects of mesenchymal stem cells on irradiated lungs via stimulating endogenous secretion of HGF and PGE2."12-4817 was used in Flow cytometry/Cell sorting to confirm that mesenchymal stem cells have the potential to limit pulmonary fibrosis after exposure to ionising irradiation."AuthorsDong LH,Jiang YY,Liu YJ,Cui S,Xia CC,Qu C,Jiang X,Qu YQ,Chang PY,Liu F4.PloS oneRetinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells."12-4817 was used in Flow cytometry/Cell sorting to investigate the activation of uveitogenic T cells via the pretreatment of retinal astrocytes with NOD2 and TLR2 ligands."AuthorsJiang G,Sun D,Kaplan HJ,Shao H5.PloS oneRetinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells."12-4817 was used in Flow cytometry/Cell sorting to investigate the activation of uveitogenic T cells via the pretreatment of retinal astrocytes with NOD2 and TLR2 ligands."AuthorsJiang G,Sun D,Kaplan HJ,Shao H |
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