CD34 Monoclonal Antibody (RAM34), eFluor 660

货号：50-0341-82,50-0341-80

规格：100µg,25μg

价格：2279,1080

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RAM34

荧光染料：eFluor 660

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, eFluor 660, eBioscience™
免疫原性：		用法：	1 µg/test(Flow）

Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD34 Antibody (50-0341-80) in Flow Staining of BALB/c bone marrow cells with Anti-Mouse CD117 (c-Kit) FITC (Product # 11-1171-82) and 1.0 µg of Anti-Mouse CD34 eFluor® 660. Lineage negative cells using the Mouse Hematopoietic Lineage eFluor® 450 Cocktail (Product # 88-7772-72) were used for analysis.

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参考文献：

1. Cell and bioscience14-3-3ζ loss leads to neonatal lethality by microRNA-126 downregulation-mediated developmental defects in lung vasculature.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Stem cells (Dayton, Ohio)CD34 promotes satellite cell motility and entry into proliferation to facilitate efficient skeletal muscle regeneration.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. International journal of radiation oncology, biology, physicsImproved intratumoral oxygenation through vascular normalization increases glioma sensitivity to ionizing radiation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. The Journal of cell biologyIntegrin-linked kinase is required for epidermal and hair follicle morphogenesis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. PloS oneZoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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