| Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.OncoimmunologyTumor-infiltrating Tim-3+ T cells proliferate avidly except when PD-1 is co-expressed: Evidence for intracellular cross talk."25045842 was used in flow cytometry to study the role of PD-1 and Tim-3 crosstalk in the regulation of antitumor T cell responses"Authors Li J,Shayan G,Avery L,Jie HB,Gildener-Leapman N,Schmitt N,Lu BF,Kane LP,Ferris RL2.Journal of immunology (Baltimore, Md. : 1950)The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects."25-0458 was used in Flow cytometry/Cell sorting to study the role of LAG-3 on T cell functionality during persistent HIV-1 infection."Authors Tian X,Zhang A,Qiu C,Wang W,Yang Y,Qiu C,Liu A,Zhu L,Yuan S,Hu H,Wang W,Wei Q,Zhang X,Xu J3.Human immunologyEffect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates."25-0458 was used in Flow cytometry/Cell sorting to show that relative to term, prematurity and chorioamnionitis decrease Treg cell functionality without affecting their frequency or phenotype."Authors Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA4.BMC infectious diseasesCytotoxic response persists in subjects treated for tuberculosis decades ago."25-0458 was used in Flow cytometry/Cell sorting to evaluate the influence of various anti-TB therapeutic schemes on the immunological profiles of residual cytotoxic T lymphocytes."Authors Savolainen LE,Koskivirta P,Kantele A,Valleala H,Pusa L,Tuompo R,Westerlund-Wikström B,Tuuminen T5.Infection and immunityHumanized mice, a new model to study the influence of drug treatment on neonatal sepsis."25-0458 was used in Flow cytometry/Cell sorting to investigate the use of humanised mice as a model of GBS-induced sepsis."Authors Ernst W,Zimara N,Hanses F,Männel DN,Seelbach-Göbel B,Wege AK |
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