Rabbit anti-Human IgG2 Recombinant Secondary Antibody/兔抗人IgG2重组二抗

货号：SA5-10203

规格：100 µg

价格：2732

产品类型：二抗

品牌：Thermo Fisher

抗原：Peptide corresponding to Human IgG2.

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	50-200 ng/well (Capture), 0.05-0.2 µg/mL (Detect)(ELISA)；0.5-2 µg/mL(ICC)；0.5-2 µg/mL(IF)； 0.5-2 µg/mL(IHC)

产品详细信息This antibody reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据：

Human IgG2 Secondary Antibody (SA5-10203) in ELISA ELISA of human immunoglobulins shows Rabbit Anti-Human IgG2 Secondary Antibody (Product # SA5-10203) only reacts to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of Rabbit Anti-Human IgG2 Secondary Antibody (Product # SA5-10203) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Human IgG2 Secondary Antibody (SA5-10203) in ELISAA titer ELISA using Rabbit Anti-Human IgG2 Secondary Antibody (Product # SA5-10203). The plate was coated with different amounts of human IgG2. A serial dilution ofRabbit Anti-Human IgG2 Secondary Antibody (Product # SA5-10203) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Human IgG2 Secondary Antibody (SA5-10203) in ELISA Detection of human IgG2 in monkey serum. Sandwich ELISA using Rabbit anti-Human IgG2 Secondary Antibody (Product # SA5-10203) as the capture antibody, and biotinylated Rabbit anti-Human IgG Secondary Antibody (Product # SA5-10224) as the detection antibody, followed by a HRP conjugated streptavidin.

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技术参数

产品应用 ELISA;ICC;IF;IHC

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