Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 800 4X PEG/山羊抗小鼠Ig(H+L)二抗，DyLight 800 4X PEG

货号：SA5-35521

规格：1 mL

价格：2335

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Purified Mouse IgG, whole molecule

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

抗体类型:	荧光二抗	同型对照：
浓度：	1 mg/mL	用法：	1:100 - 1:2000 (IF);Assay Dependent (IP);1:100 - 1:2000 (IHC);1:10,000-1:30,000 (WB)

产品详细信息Product SA5-35521 reacts with the heavy chains of Mouse IgG and with the light chains common to most Mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol without and store at -20°C. Note: not compatible with sodium azide. Mole Dye/Mole Protein Ratio is lot-dependent.DyLight 800-4xPEG has an excitation (nm) of 783 nm (Amax) and an emission (nm) of 797 nm (Emax) in PBS. The emission spectra for DyLight™ 800-4xPEG conjugate match the principal output wavelengths of most common fluorescence instrumentation. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Secondary Antibody (SA5-35521) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected using Goat anti-Mouse IgG (H and L) Secondary Antibody, DyLight 800 4X PEG (Product # SA5-35521) at dilutions 1:10,000 (Fig. 1) and 1:30,000 (Fig. 2). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences). Mouse IgG (H+L) Secondary Antibody (SA5-35521) in WBWestern blot analysis of DNA-PKcs was performed by loading 30 µg of MiaPaCa-2 lysate (lane 1), 30 µg of OVCAR-8 lysate (lane 2) and 30 µg of MDA-MB-231 lysate (lane 3) in reducing sample buffer and Page Ruler Protein Ladder (Product # 26616) onto a 6% polyacrylamide gel. Proteins were transferred to PVDF membrane using the wet transfer method. Membrane was blocked in Super Block Blocking Buffer (Product # 37515). DNA-PKcs was detected at approximately 469 kDa using a DNA-PKcs monoclonal antibody (Product # MA5-13244) at a dilution of 1:1000 in 5% BSA, followed by a goat anti-mouse IgG DyLight 800 conjugated secondary antibody (Product # SA5-35521) at a dilution of 1:7500. Data courtesy of Antibody Data Exchange Program.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.PloS oneLTBP-2 Has a Single High-Affinity Binding Site for FGF-2 and Blocks FGF-2-Induced Cell Proliferation.2.Methods (San Diego, Calif.)A quantitative assay to monitor HSV-1 ICP0 ubiquitin ligase activity in vitro.

技术参数

产品应用 IF;IHC;IP;WB

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21800.htm