Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Biotin-XX/山羊抗小鼠IgG1交叉吸附二抗，生物素-XX

货号：A10519

规格：500 µg

价格：1832

产品类型：二抗

品牌：Thermo Fisher

抗原：IgG gamma 1

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

抗体类型:	二抗	同型对照：
浓度：	2mg/mL	用法：	1:1,000-1:10,000 (WB);1-10 µg/mL (IF)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG1 Cross-Adsorbed Secondary Antibody (A10519) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of HEL 92.1.7 (Lane 1) and K-562 (Lane 2). The blots were probed with Anti CUL1 Mouse Monoclonal Antibody (Product # 32-2400, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Biotin-XX (Product # A10519) at dilutions 1:1,000 (Fig. 1), 1:5,000 (Fig. 2) and 1:10,000 (Fig. 3). A 89 kDa band corresponding to CUL1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Neuropathology and applied neurobiologyInvestigating complex I deficiency in Purkinje cells and synapses in patients with mitochondrial disease.2.Journal of cell scienceFibrillin-1 directly regulates osteoclast formation and function by a dual mechanism.3.The Journal of biological chemistryTumor-supportive and osteoclastogenic changes induced by breast cancer-derived factors are reversed by inhibition of {gamma}-secretase.

技术参数

产品应用 IF;WB;IHC(P);Misc

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