CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), FITC

货号：11-0689-41,11-0689-42

规格：25 Tests,100 Tests

价格：1038,2112

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：eBioY1/82A (Y1/82A)

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), FITC
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow)；0.125 µg/mL (ICC)；0.125 µg/mL(IF)

产品详细信息Description: The eBioY1/82 antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on it's homolgy and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.Applications Reported: This eBioY1/82A (Y1/82A) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioY1/82A (Y1/82A) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma orhistiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

数据

CD68 Antibody (11-0689-41) in FlowIntracellular staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control FITC (Product # 11-4732-42) (blue histogram) or Anti-Human CD68 FITC (purple histogram). Cells in the monocyte gate were used for analysis.

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参考文献：

1.American journal of cancer researchBaicalein suppress EMT of breast cancer by mediating tumor-associated macrophages polarization."11-0689 was used in Flow cytometry/Cell sorting to explore the potential effect of baicalein on macrophage polarisation and epithelial-mesenchymal transition of breast cancer."AuthorsZhao X,Qu J,Liu X,Wang J,Ma X,Zhao X,Yang Q,Yan W,Zhao Z,Hui Y,Bai H,Zhang S2.ALTEXIn vitro chemotaxis and tissue remodeling assays quantitatively characterize foreign body reaction."11-0689 was used in Flow cytometry/Cell sorting to investigate the material dependency of fibroblast chemotaxis and tissue remodelling mediated by material-resident macrophages."AuthorsJannasch M,Weigel T,Engelhardt L,Wiezoreck J,Gaetzner S,Walles H,Schmitz T,Hansmann J3.Scientific reportsA comparative multi-parametric in vitro model identifies the power of test conditions to predict the fibrotic tendency of a biomaterial."11-0689 was used in Flow cytometry/Cell sorting to show that biomaterial ranking obtained under native plasma test conditions showed a high predictive accuracy compared to in vivo assessments."AuthorsJannasch M,Gaetzner S,Weigel T,Walles H,Schmitz T,Hansmann J4.Scientific reportsA comparative multi-parametric in vitro model identifies the power of test conditions to predict the fibrotic tendency of a biomaterial."11-0689 was used in Flow cytometry/Cell sorting to show that biomaterial ranking obtained under native plasma test conditions showed a high predictive accuracy compared to in vivo assessments."AuthorsJannasch M,Gaetzner S,Weigel T,Walles H,Schmitz T,Hansmann J5.Bioscience reportsMicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3."11-0689 was used in Flow cytometry/Cell sorting to investigate the role of miR-720 in tumour-associated macrophages (TAMs), showing that overexpression of miR-720 leads to suppression of M2 macrophage polarization by targeting GATA3."

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