CD69 Monoclonal Antibody (H1.2F3), FITC

货号：11-0691-81,11-0691-82,11-0691-85

规格：50μg,100μg,500μg

价格：838,1215,2840

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：H1.2F3

荧光染料：FITC

类型:	流式抗体	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), FITC
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: The H1.2F3antibody has been reported for use in flow cytometric analysis.Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

数据

CD69 Antibody (11-0691-81) in FlowStaining of unstimulated (left) or overnight ConA-stimulated (right) C57BL/6 mouse splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD69 FITC (purple histogram). Total viable cells were analyzed.

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参考文献：

1.Frontiers in immunologyLenalidomide and Programmed Death-1 Blockade Synergistically Enhances the Effects of Dendritic Cell Vaccination in a Model of Murine Myeloma."11-0691 was used in Flow cytometry/Cell sorting to investigate the efficacy of dendritic cell vaccination in combination with lenalidomide and PD-1 blockade in a model of murine myeloma."AuthorsVo MC,Jung SH,Chu TH,Lee HJ,Lakshmi TJ,Park HS,Kim HJ,Rhee JH,Lee JJ2.Frontiers in immunologyThe Combination Therapy of Dietary Galacto-Oligosaccharides With Budesonide Reduces Pulmonary Th2 Driving Mediators and Mast Cell Degranulation in a Murine Model of House Dust Mite Induced Asthma."11-0691 was used in Flow cytometry/Cell sorting to compare the efficacy of budesonide or different doses of galacto-oligosaccharides alone or with a combination therapy of budesonide and galacto-oligosaccharides on house dust mite-allergic responses in mice."AuthorsVerheijden KAT,Braber S,Leusink-Muis T,Jeurink PV,Thijssen S,Kraneveld AD,Garssen J,Folkerts G,Willemsen LEM3.Nature communicationsNF1 heterozygosity fosters de novo tumorigenesis but impairs malignant transformation."11-0691 was used in Flow cytometry/Cell sorting to study the role of the neurofibromatosis type 1 tumour suppressor gene in de novo tumorigenesis and malignant transformation."AuthorsBrosseau JP,Liao CP,Wang Y,Ramani V,Vandergriff T,Lee M,Patel A,Ariizumi K,Le LQ4.Frontiers in immunologyLenalidomide and Programmed Death-1 Blockade Synergistically Enhances the Effects of Dendritic Cell Vaccination in a Model of Murine Myeloma.5.Nature communicationsOncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion."Published figure using CD69 monoclonal antibody (Product # 11-0691-82) in Flow Cytometry"

技术参数

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