IL-4 Monoclonal Antibody (11B11), Functional Grade, eBioscience™/IL-4 单克隆 (11B11), 功能抗体, eBioscience™

货号：16-7041-81,16-7041-85,16-7041-38,16-7041-95

规格：50 µg,500 µg,5 mg,10 mg

价格：1081,3618,24932,30248

产品类型：流式抗体

品牌：eBioscience

抗原：IL-4

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：11B11

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/mL（ELISA）;Assay-Dependent(功能检测）

产品详细信息Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surfaceMHC class II and CD23.Applications Reported: This 11B11 antibody has been reported for use in cytokine neutralization, ELISPOT, and ELISA cap.Applications Tested: The Functional Grade Purified 11B11 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-4 bioactivity.The 11B11 antibody at 0.125 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-4 in a CTLL-2 cell proliferation assay. Please refer to Best Protocols for more information.The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-4 (IL-4) in combination with the biotinylated BVD6-24G2 (cat. 13-7042) antibody for detection and recombinant mouse IL-4 (cat. 14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.仅用于科研。不用于诊断过程。

数据

IL-4 Antibody (16-7041-81) in ELISASplenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL-4 ELISPOT assay. Control well is medium alone.

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参考文献：

流式1.Scientific reportsPaeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation."16-7041 was used in Flow cytometry/Cell sorting to investigate the effect Paeoniflorin has on Th cells and dendritic cells in a mouse MS model."AuthorsZhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H2.BloodRecent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage."16-7041 was used in Flow cytometry/Cell sorting to investigate the relationships between recent thymic emigrants and T-helper type 1 and 2 cells."AuthorsHendricks DW,Fink PJ功能检测3.The Journal of biological chemistryNADPH Oxidases Are Essential for Macrophage Differentiation."16-7041 was used in Functional assays to investigate the role of NOX1 and NOX2 in macrophage differentiation and tumor progression."AuthorsXu Q,Choksi S,Qu J,Jang J,Choe M,Banfi B,Engelhardt JF,Liu ZG4.Cell reportsSingle-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis."16-7041 was used in Functional assays to demonstrate the production of the steroid pregnenolone by Th2 cells in vitro and in vivo in a helminth infection model."AuthorsMahata B,Zhang X,Kolodziejczyk AA,Proserpio V,Haim-Vilmovsky L,Taylor AE,Hebenstreit D,Dingler FA,Moignard V,Göttgens B,Arlt W,McKenzie AN,Teichmann SAELISA5.Nature communicationsIL-15-dependent balance between Foxp3 and RORγt expression impacts inflammatory bowel disease."16-7041 was used in an ELISA assay to demonstrate that impaired delivery of IL-15 to CD4+ T cells in the colon down-modulates Foxp3 expression and enhances RORγt expression."AuthorsTosiek MJ,Fiette L,El Daker S,Eberl G,Freitas AA

技术参数

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