Arginase 1 Monoclonal Antibody (A1exF5), PE
货号：12-3697-80,12-3697-82
规格：25μg,100μg
价格：1174,2752
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：PE
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), PE |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both the standard intracellular protocols and the Foxp3/Transcription Factor Staining Buffer Set.Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser靶标信息Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia. |
| 数据 |
| Arginase 1 Antibody (12-3697-82) in FlowNormal human lysed whole blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.25 µg of Arginase 1 Monoclonal Antibody, PE. Cells were co-stained with CD16 Monoclonal Antibody, FITC (Product # 11-0168-42) and CD193 Monoclonal Antibody, APC (Product # 17-1939-42). Cells in the granulocyte gate (left panel) were used for analysis and were gated on either the CD193+ population (blue histogram) or the CD16+ population (purple histogram). |
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