| 产品介绍 | | |
| Rabbit anti Human CXCL9 polyclonal antinbodyrecognizes human CXCL9, otherwise known as MIG (monokine induced by Interferon-gamma), a member of the CXC family of chemokine receptors, secreted by interferon stimulated monocytes, macrophages (Mulleret al.2010) and endothelial cells (Looset al.2006). CXCL9 signals through binding toCD183(CXCR3).CXCL9 acts as a Th1 (type 1 helper T) cell chemoattractant, and plays a role in the growth, activation and movement of cells associated withimmune and inflammatory responses, and in tumou growth inhibition and angiogenesis (Whitinget al.2004) . CXCL9 is active againstE.coli, S.aureus, L.monocytogenes(Coleet al.2001) andS.pyogenes(Egestenet al.2007). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
Antiserum Preparation | Antiserum to human CXCL9 were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
Immunogen | Recombinant human CXCL9 |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
| Functional Assays | √ | 5.0 | 7.0ug/ml |
| Immunohistology - Paraffin 1 | √ | | |
| Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 数据 |
| Formalin fixed, paraffin embedded human renal tumor with parenchymal tissue stained with Rabbit anti Human CXCL9 (AHP1864) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0) |
| 相关试剂 |
| Description | Product Code | Applications | Pack Size |
| Antigen Retrieval Buffer, pH8.0 | BUF025A | IHC(P) | 500 mL |
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB | 0.5 mL |
| 参考文献 |
| | | |
| 1. Ciccia, F.et al.(2014) Macrophage phenotype in the subclinical gut inflammation of patients with ankylosing spondylitis. Rheumatology (Oxford). 53 (1): 104-13. |
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