Rabbit anti Human CD120b:Biotin
货号：AHP1833B
规格：0.1 mg
价格：4208
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：CD120b
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Biotin
| 产品介绍 | | |
| Rabbit anti human CD120brecognizes the soluble form of human CD120b, otherwise known as sTNF receptor type II (sTNFR1I), a member of the tumour necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE.CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFR1I acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Biotin - lyophilized |
Antiserum Preparation | Antiserum to human CD120b were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 0.1mg/ml after reconstitution |
Immunogen | Recombinant human CD120b(PHP215) |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Reconstitution | Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.25 | 1.0ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- ELISA
- This biotinylated human CD120b antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with apurified human CD120b antibody(AHP1833) as the capture reagent andrecombinant human CD120b(PHP215) as the standard.
- Western Blotting
- This biotinylated human CD120b antibody may used in Western Blotting applications under either reducing or non-reducing conditions withrecombinant human CD120b(PHP215) as the positive control.
|
| 数据 |
Immunofluorescent staining of PC3 cells with Rabbit anti Human CD120b antibody (AHP1833), green. Counterstained with actin, red and nuclei, blue |
技术参数 产品应用 ELISA;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;WB
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